Catalog Number - 85118-05P-2

The 85118‑05P‑2 Cogent RP C18 ABX™ UHPLC Column is engineered for robust separations of compounds with extreme pKa values, offering stable performance across a wide pH range (2–12) using a specially end‑capped C18 bonded phase.

Manufactured with bulky end‑capping and high‑purity silica, this phase minimizes silanol interactions, improving peak shape for acidic, basic, and polar analytes under challenging mobile phase conditions.

✅ Ideal for USP L1 methods involving strong acids or bases

50 mm Length – Fast UHPLC & Method Scouting

• Designed for rapid separations and short analysis times

• Ideal for high‑throughput UHPLC workflows

• Reduces solvent consumption and run time

• Excellent for method development and fast screening

✅ Preferred for LC‑MS method scouting and rapid optimization

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Optimized for UHPLC and LC‑MS systems

• Enhances MS sensitivity through reduced solvent load

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires UHPLC systems capable of stable low‑flow delivery

1.8 µm Particle – Maximum Efficiency & Peak Capacity

• Ultra‑high efficiency compared to 3 µm and larger particles

• Produces sharper peaks and improved resolution

• Increases peak capacity for complex mixtures

✅ Ideal for advanced UHPLC and LC‑MS analytical workflows

⚠️ Higher backpressure expected — UHPLC system required

Wide pH Stability (pH 2–12) – Extreme Conditions Capability

• Designed for high and low pH mobile phases

• Suitable for:

  • Strongly acidic compounds

  • Strongly basic compounds

• Reduces degradation under aggressive method conditions

✅ Enables method flexibility not achievable with standard C18 phases

Why Choose RP C18 ABX™ Columns

Extreme pH Ruggedness

• Stable performance across broad pH range

• Ideal for challenging analytes and harsh mobile phases

Improved Peak Shape for Acids & Bases

• Specialized end‑capping reduces secondary interactions

• Enhances chromatographic reproducibility

Method Development Advantage

• Faster runs with lower solvent consumption

• Increases throughput and lab efficiency

✅ Excellent choice for new method development and optimization

System & Sample Considerations

• ⚠️ Requires UHPLC system capable of high pressure operation

• Recommended sample preparation:

  • 👉 Use 0.2 µm syringe filters for sample preparation before injection

✅ Helps prevent blockage and maintain column performance

Typical Applications

• LC‑MS and UHPLC analysis

• Acidic and basic compound separations

• Pharmaceutical method development

• Stability‑indicating methods

• Peptides and polar compounds

• Complex mixture analysis under extreme pH

Important Usage Notes

• Designed for UHPLC systems only

• Best suited for extreme pH mobile phases (pH 2–12)

• Ideal for fast analysis and LC‑MS workflows

• Monitor backpressure to ensure system compatibility